Bacteriophage Ecology Group

Bacteriophage Ecology Group Bacteriophage Ecology Group

Adsorption

The sum of processes culminating in attachment of a phage to a bacterium (and possibly including phage nucleic acid translocation into a bacterium's cytoplasm as well).

 CAUTION: POTENTIALLY AMBIGUOUS TERM 

Narrowly defined the term adsorption can be used synonymously with attachment but more broadly includes all of the steps associated with converting a free phage into an irreversibly bacterium-attached phage and/or phage-infected bacterium.

Note that the process of adsorption can be divided into movement, encounter, attachment, and irreversible attachment steps. Typically diffusion will occur that is followed by random encounter between a phage and a potential bacterial host. If phage adsorption proteins possess sufficient affinity for phage receptors found on the host surface then at least a reversible attachment may occur. Irreversible attachment involves stronger interactions between the virion particle and the bacterial surface as well as irreversible conformational changes in the virion that are associated with phage genome translocation into the cytoplasm of the bacterial cell. See also adsorption rate constant. This is the definition from Adams (1959, InterScience, p. 439): "Attachment of phage particle to bacterium, irreversible unless otherwise stated."

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