Bacteriophage Ecology Group

Bacteriophage Ecology Group Bacteriophage Ecology Group

Lysate

 

A bacterial culture that has been subject to phage-induced lysis and that typically contains so-released free phages.

The generation of lysates is the middle step of phage stock production, preceded by phage propagation to higher titers and followed by either phage use or various purification steps prior to use.

The term "crude lysate" refers to such cultures so long as they have been subject to either no or only minimal phage purification and, as a consequence, crude lysates can contain substantial amounts of bacterial lysis products, such as endotoxin.

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